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mouse c3a elisa kit  (Novus Biologicals)


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    Novus Biologicals mouse c3a elisa kit
    Mouse C3a Elisa Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+complement+c3a+elisa+kit/Mouse+Complement+C3a+ELISA+Kit+(Colorimetric)/pm41963079-229-15-19
    Average 94 stars, based on 12 article reviews
    mouse c3a elisa kit - by Bioz Stars, 2026-09
    94/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cells
    Article Snippet: Fixable Viability Dye eFluor780 , eBioscience , Cat # 65-0865-14. .. Mouse complement C3a ELISA kit , Novus Biologicals , Cat # NBP2-70037. .. FTY720 , Sigma-Aldrich , Cat # SML0700.

    Article Title: Anti-inflammatory actions of Pentosan polysulfate sodium in a mouse model of influenza virus A/PR8/34-induced pulmonary inflammation.
    Article Snippet: Data was exported and analyzed using the FCAP Array v3.0 software (BD Biosciences). .. Complement proteins were quantified using single ELISA kits (Novus Biologicals Mouse Complement C3a ELISA kit, Cat#NBP2-70037; Thermo Fisher Mouse Complement C5a ELISA kit, Cat#EHMC; Hycult Biotech Mouse Complement C3b ELISA kit, Cat#HK216-01; Biomatik mouse Terminal Complement Complex C5b9 (Cat#EKU10320); Cusabio mouse Factor B (Complement Factor Bb; CF-Bb) Cat#CSB-E04695m); R&D Systems mouse soluble ICAM-1 (sICAM-1) Quantikine Cat#MIC100) according to manufacturer’s instructions. .. Optical density values (450nm and 570nm) were read on a POLARStar Omega plate reader (BMG Labtech) and data exported to .csv files using the manufacturer’s proprietary software.

    Article Title: Anti-inflammatory actions of Pentosan polysulfate sodium in a mouse model of influenza virus A/PR8/34-induced pulmonary inflammation
    Article Snippet: Data was exported and analyzed using the FCAP Array v3.0 software (BD Biosciences). .. Complement proteins were quantified using single ELISA kits (Novus Biologicals Mouse Complement C3a ELISA kit, Cat#NBP2-70037; Thermo Fisher Mouse Complement C5a ELISA kit, Cat#EHMC; Hycult Biotech Mouse Complement C3b ELISA kit, Cat#HK216-01; Biomatik mouse Terminal Complement Complex C5b9 (Cat#EKU10320); Cusabio mouse Factor B (Complement Factor Bb; CF-Bb) Cat#CSB-E04695m); R&D Systems mouse soluble ICAM-1 (sICAM-1) Quantikine Cat#MIC100) according to manufacturer’s instructions. .. Optical density values (450nm and 570nm) were read on a POLARStar Omega plate reader (BMG Labtech) and data exported to .csv files using the manufacturer’s proprietary software.

    Article Title: Impact of Pre-existing Anti-polyethylene Glycol Antibodies on the Pharmacokinetics and Efficacy of a COVID-19 mRNA Vaccine (Comirnaty) In Vivo
    Article Snippet: .. Mouse blood was collected after vaccination at 15 min, 3 h, 8 h, and 24 h. The complement C3a concentration in 50-fold diluted mouse plasma was measured using Mouse Complement C3a ELISA Kit (NBP2-70037, Novus Biologicals) according to the manufacturer’s instructions. .. Plasma concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein were measured using SARS-CoV-2 Spike Protein Titer Assay Kit (RAS-A020, ACROBiosystems).

    Concentration Assay:

    Article Title: Impact of Pre-existing Anti-polyethylene Glycol Antibodies on the Pharmacokinetics and Efficacy of a COVID-19 mRNA Vaccine (Comirnaty) In Vivo
    Article Snippet: .. Mouse blood was collected after vaccination at 15 min, 3 h, 8 h, and 24 h. The complement C3a concentration in 50-fold diluted mouse plasma was measured using Mouse Complement C3a ELISA Kit (NBP2-70037, Novus Biologicals) according to the manufacturer’s instructions. .. Plasma concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein were measured using SARS-CoV-2 Spike Protein Titer Assay Kit (RAS-A020, ACROBiosystems).

    Clinical Proteomics:

    Article Title: Impact of Pre-existing Anti-polyethylene Glycol Antibodies on the Pharmacokinetics and Efficacy of a COVID-19 mRNA Vaccine (Comirnaty) In Vivo
    Article Snippet: .. Mouse blood was collected after vaccination at 15 min, 3 h, 8 h, and 24 h. The complement C3a concentration in 50-fold diluted mouse plasma was measured using Mouse Complement C3a ELISA Kit (NBP2-70037, Novus Biologicals) according to the manufacturer’s instructions. .. Plasma concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein were measured using SARS-CoV-2 Spike Protein Titer Assay Kit (RAS-A020, ACROBiosystems).



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    Image Search Results


    Inhibition of C3aR ameliorates rotenone-induced neurodegeneration, synaptic loss, α-synuclein phosphorylation and cognitive deficits in mice. C57BL/6 mice were treated with rotenone for 3 weeks to establish PD models, with 30 min of SB290157 (C3aR inhibitor, 1 mg/kg, i.p.) administration after each rotenone injection. (A) Concentrations of C3a in the hippocampus of Con and Rot mice, measured by enzyme-linked immunosorbent assay (ELISA). n = 6. (B) Representative Western blot images and densitometric quantification of C3aR protein levels in the hippocampus and rotenone mice. n = 4. (C) The mRNA levels of C3aR in vehicle and rotenone mice. (D) Representative images of double-immunofluorescence staining with C3aR and Iba-1 or Neu-N antibodies and (E, F) the quantification of C3aR + Iba-1 + and C3aR + Neu-N + cells in vehicle and rotenone mice. n = 3. (G) The quantification of Neu-N + cell number and (H) optical density of PSD95 staining in rotenone mice with or without C3aR inhibitor treatment. (I) The representative images of Neu-N and PSD95 staining in rotenone mice with or without C3aR inhibitor treatment. n = 6. (J, K) Representative bands of Neu-N, PSD95 and ser129-phosphorylated α-synuclein and the quantification of blots in rotenone mice with or without C3aR inhibitor treatment. n = 4. (L) Representative images of ser129-phosphorylated α-synuclein and (M) the quantification of staining density in rotenone mice with or without C3aR inhibitor treatment. n = 3. (N–Q) Escape latency, traveled distance, first platform crossing latency and platform crossing number in rotenone mice with or without C3aR inhibitor treatment. n = 15; ∗ p < 0.05, ∗∗ p < 0.01 for comparison between Rot & Rot + C3aR inhibitor groups; Scale bar = 50 μm.

    Journal: Redox Biology

    Article Title: The C3–C3aR axis drives rotenone-induced cognitive damage via synaptic engulfment, dark microglia and PANoptosis

    doi: 10.1016/j.redox.2026.104062

    Figure Lengend Snippet: Inhibition of C3aR ameliorates rotenone-induced neurodegeneration, synaptic loss, α-synuclein phosphorylation and cognitive deficits in mice. C57BL/6 mice were treated with rotenone for 3 weeks to establish PD models, with 30 min of SB290157 (C3aR inhibitor, 1 mg/kg, i.p.) administration after each rotenone injection. (A) Concentrations of C3a in the hippocampus of Con and Rot mice, measured by enzyme-linked immunosorbent assay (ELISA). n = 6. (B) Representative Western blot images and densitometric quantification of C3aR protein levels in the hippocampus and rotenone mice. n = 4. (C) The mRNA levels of C3aR in vehicle and rotenone mice. (D) Representative images of double-immunofluorescence staining with C3aR and Iba-1 or Neu-N antibodies and (E, F) the quantification of C3aR + Iba-1 + and C3aR + Neu-N + cells in vehicle and rotenone mice. n = 3. (G) The quantification of Neu-N + cell number and (H) optical density of PSD95 staining in rotenone mice with or without C3aR inhibitor treatment. (I) The representative images of Neu-N and PSD95 staining in rotenone mice with or without C3aR inhibitor treatment. n = 6. (J, K) Representative bands of Neu-N, PSD95 and ser129-phosphorylated α-synuclein and the quantification of blots in rotenone mice with or without C3aR inhibitor treatment. n = 4. (L) Representative images of ser129-phosphorylated α-synuclein and (M) the quantification of staining density in rotenone mice with or without C3aR inhibitor treatment. n = 3. (N–Q) Escape latency, traveled distance, first platform crossing latency and platform crossing number in rotenone mice with or without C3aR inhibitor treatment. n = 15; ∗ p < 0.05, ∗∗ p < 0.01 for comparison between Rot & Rot + C3aR inhibitor groups; Scale bar = 50 μm.

    Article Snippet: The C3a ELISA kit was purchased from CUSABIO (CSB- E08511 m, Houston, TX, USA), and TUNEL assay kit was provided by KeyGEN (KGA7073-1, Nanjing, China.

    Techniques: Inhibition, Phospho-proteomics, Injection, Enzyme-linked Immunosorbent Assay, Western Blot, Double Immunofluorescence Staining, Staining, Comparison